Potencial antitumoral in vitro do extrato vegetal de Baccharis dracunculifolia
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Data
2023-12
Tipo de documento
Artigo Científico
Título da Revista
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Área do conhecimento
Modalidade de acesso
Acesso fechado
Editora
Autores
GIOVANNINI, Lucas Adrião
Orientador
MARIA, Durvanei Augusto
Coorientador
MALONE, Camila F. Silva
Resumo
O melanoma é um câncer de pele com o pior prognóstico devido a ocorrência de metástase intra linfática em 5 a 10%, promovendo resistência terapêutica. A utilização de novas terapias e o prognóstico precoce tem mostrado bons resultados em relação aos tratamentos quimioterápicos convencionais. A Dacarbazina é um fármaco indicado no tratamento de melanoma metastático, disponível na rede pública de saúde, porém trata-se de uma droga que provoca danos ao DNA, resultando em apoptose e inibição proliferativa. Em decorrência dos efeitos colaterais do Dacarbazina, alternativas de origem vegetal vêm sendo pesquisadas. Pesquisas recentes demonstram que extratos vegetais contêm uma variedade de compostos bioativos, dos quais, alguns expressam potencial antitumoral. Estudos prévios sugeriram que Baccharis dracunculifolia possui propriedades antiproliferativas In vitro. Desta forma, objetivou-se neste estudo avaliar o efeito do extrato vegetal de Baccharis dracunculifolia em células tumorais de melanoma, a fim de conhecer sua eficácia e os mecanismos de sua ação antitumoral. A relevância deste estudo reside na possibilidade compreender os processos biológicos e mecanismos do extrato de Baccharis dracunculifolia, além de oferecer uma alternativa terapêutica e farmacológica. Para tal, serão realizados ensaios In vitro, utilizando a linhagem celular de melanoma humano SK-Mel-28 e outra de fibroblasto humano FN-1, submetidas ao extrato vegetal. Os resultados demonstraram efetividade e seletividade do extrato de B. dracunculifolia. Ocorrem modificações na distribuição das populações nas fases do ciclo celular, com redução das células em G0/G1 e parada proliferativa em G2M. O tratamento reduz significantemente o potencial elétrico mitocondrial em células tumorais. O conjunto de resultados mostra o potencial inibitório da resposta proliferativa e seletiva do extrato de B. dracunculifolia para as células de melanoma em comparação às células normais humanas. Os dados sugerem que o extrato vegetal de B. dracunculifolia é um possível candidato para um tratamento adjuvante contra câncer de pele.
Melanoma is a skin cancer with the worst prognosis due to the occurrence of intra-lymphatic metastasis in 5 to 10%, promoting therapeutic resistance. The use of new therapies and early prognosis has shown good results in relation to conventional chemotherapy treatments. Dacarbazine is a drug indicated for the treatment of metastatic melanoma, available in the public health network, but it is a drug that causes DNA damage, resulting in apoptosis and proliferative inhibition. Due to the side effects of Dacarbazine, alternatives of plant origin have been researched. Recent research demonstrates that plant extracts contain a variety of bioactive compounds, some of which express antitumor potential. Previous studies have suggested that Baccharis dracunculifolia has antiproliferative properties in vitro. Therefore, the objective of this study was to evaluate the effect of Baccharis dracunculifolia plant extract on melanoma tumor cells, to understand its effectiveness and the mechanisms of its antitumor action. The relevance of this study lies in the possibility of understanding the biological processes and mechanisms of Baccharis dracunculifolia extract, in addition to offering a therapeutic and pharmacological alternative. To this end, In vitro tests will be carried out, using the human melanoma cell line SK-Mel-28 and another human fibroblast cell line FN-1, subjected to the plant extract. The results demonstrated the effectiveness and selectivity of the B. dracunculifolia extract. There are changes in the distribution of populations in the phases of the cell cycle, with a reduction in cells in G0/G1 and proliferative arrest in G2M. The treatment significantly reduces mitochondrial electrical potential in tumor cells. The set of results shows the inhibitory potential of the proliferative and selective response of B. dracunculifolia extract to melanoma cells compared to normal human cells. The data suggest that the plant extract of B. dracunculifolia is a possible candidate for an adjuvant treatment against skin cancer.
Melanoma is a skin cancer with the worst prognosis due to the occurrence of intra-lymphatic metastasis in 5 to 10%, promoting therapeutic resistance. The use of new therapies and early prognosis has shown good results in relation to conventional chemotherapy treatments. Dacarbazine is a drug indicated for the treatment of metastatic melanoma, available in the public health network, but it is a drug that causes DNA damage, resulting in apoptosis and proliferative inhibition. Due to the side effects of Dacarbazine, alternatives of plant origin have been researched. Recent research demonstrates that plant extracts contain a variety of bioactive compounds, some of which express antitumor potential. Previous studies have suggested that Baccharis dracunculifolia has antiproliferative properties in vitro. Therefore, the objective of this study was to evaluate the effect of Baccharis dracunculifolia plant extract on melanoma tumor cells, to understand its effectiveness and the mechanisms of its antitumor action. The relevance of this study lies in the possibility of understanding the biological processes and mechanisms of Baccharis dracunculifolia extract, in addition to offering a therapeutic and pharmacological alternative. To this end, In vitro tests will be carried out, using the human melanoma cell line SK-Mel-28 and another human fibroblast cell line FN-1, subjected to the plant extract. The results demonstrated the effectiveness and selectivity of the B. dracunculifolia extract. There are changes in the distribution of populations in the phases of the cell cycle, with a reduction in cells in G0/G1 and proliferative arrest in G2M. The treatment significantly reduces mitochondrial electrical potential in tumor cells. The set of results shows the inhibitory potential of the proliferative and selective response of B. dracunculifolia extract to melanoma cells compared to normal human cells. The data suggest that the plant extract of B. dracunculifolia is a possible candidate for an adjuvant treatment against skin cancer.
Palavras-chave
Baccharis dracunculifolia, Bioativo natural, Melanoma humano, Dacarbazina