Impacto da expressão do CD10 nos neutrófilos maduros em pacientes com Leucemia Linfoblástica Aguda B e sua correlação com infecções
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Data
2023-12
Tipo de documento
Artigo Científico
Título da Revista
ISSN da Revista
Título de Volume
Área do conhecimento
Modalidade de acesso
Acesso aberto
Editora
Autores
SANTOS, Júlia Salvi dos
Mariela Graneiro
SILVA
Fabiane
Fernanda Hermes
Alessandra Aparecida
Mariana Bohns
Liane Esteves
Orientador
BASSUINO, Mauricio Sprenger
Coorientador
FARIAS, Mariela Graneiro
Resumo
Introdução: A ausência de expressão do CD10 nos neutrófilos maduros em pacientes com Leucemia Linfoblástica aguda de Linhagem B (LLA-B) pode estar associada com casos de infecções virais, podendo ocorrer também uma pancitopenia, alterações moleculares e citogenéticas. Essas alterações estão fortemente associadas com um pior prognóstico da doença. Objetivo: Avaliar a expressão dos marcadores de neutrófilos maduros em pacientes com LLA-B para correlacionar com demais testes laboratoriais como indicativos de infecção. Método: Neste estudo transversal, retrospectivo, as amostras de pacientes com diagnóstico de LLA B foram analisadas por citometria de fluxo, identificando a expressão de marcadores de neutrófilos maduros (CD10, CD13, CD33, CD123, CD15/CD65) de acordo com seu MFI (mean fluorescence intensity) e associadas a outros exames laboratoriais como PCR (Proteína C Reativa), hemocultura e parâmetros do hemograma (contagem de leucócitos e bastões). Resultados: Na avaliação da associação entre a expressão dos marcadores de neutrófilos estudados com a contagem de leucócitos, encontramos uma diferença significativa, notou-se que o marcador mais alterado foi o CD10, que tem seu MFI reduzido quando comparado com as altas contagens de leucócitos (>12.0x109/L). Não foi encontrada associação significativa entre contagem de leucócitos com os demais marcadores de neutrófilos. A associação com os demais parâmetros como contagem de bastões, PCR e hemocultura não foi significativa. Conclusão: A redução da expressão de CD10 pode estar fortemente associada com altas contagens de leucócitos, nos levando a
considerar uma correlação positiva com infecções, porém é necessário novos estudos para confirmação destes resultados.
Introduction: The non expression of Cd10 on mature neutrophils in line B acute lymphoblastic leukemia patients (LLA-B) may be associated with viral infections, with occurrence of pancytopenia, molecular and cytogenetic alterations. Those alterations are strongly associated with a bad prognosis. Objective: Evaluate the expression of mature neutrophils markers on LLA-B patients to correlate with other laboratory tests, like infection analysis. Method: In this cross-sectional, retrospective study, samples from patients diagnosed with LLA-B were analyzed by flow cytometry, identifying the expression of mature neutrophil markers (CD10, CD13, CD33, CD123, CD15/CD65) according to the MFI (mean fluorescence intensity) and associated with laboratories exams, like PCR (C-reactive protein), blood culture and blood count parameters (leukocyte and rod counts). Results: When evaluating the association between the expression of trained neutrophil markers and the leukocyte count, we found a significant difference. It was noted that the most altered marker was CD10, which has its MFI reduced when compared to high leukocyte counts (> 12.0x109/L). No significant association was found between leukocyte count and other neutrophil markers. The association with other parameters; stick count, PCR and blood culture were not significant. Conclusion: The reduction in the CD10 marker may be strongly associated with high leukocyte counts, leading us to consider a positive correlation with infections. However, further studies are needed to confirm these results.
Introduction: The non expression of Cd10 on mature neutrophils in line B acute lymphoblastic leukemia patients (LLA-B) may be associated with viral infections, with occurrence of pancytopenia, molecular and cytogenetic alterations. Those alterations are strongly associated with a bad prognosis. Objective: Evaluate the expression of mature neutrophils markers on LLA-B patients to correlate with other laboratory tests, like infection analysis. Method: In this cross-sectional, retrospective study, samples from patients diagnosed with LLA-B were analyzed by flow cytometry, identifying the expression of mature neutrophil markers (CD10, CD13, CD33, CD123, CD15/CD65) according to the MFI (mean fluorescence intensity) and associated with laboratories exams, like PCR (C-reactive protein), blood culture and blood count parameters (leukocyte and rod counts). Results: When evaluating the association between the expression of trained neutrophil markers and the leukocyte count, we found a significant difference. It was noted that the most altered marker was CD10, which has its MFI reduced when compared to high leukocyte counts (> 12.0x109/L). No significant association was found between leukocyte count and other neutrophil markers. The association with other parameters; stick count, PCR and blood culture were not significant. Conclusion: The reduction in the CD10 marker may be strongly associated with high leukocyte counts, leading us to consider a positive correlation with infections. However, further studies are needed to confirm these results.
Palavras-chave
Citometria de Fluxo, D10, Procalcitonina, Imunofenotipagem