O uso da biologia molecular como diagnóstico diferencial e específico de arboviroses
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Data
2022-12-12
Tipo de documento
Artigo Científico
Título da Revista
ISSN da Revista
Título de Volume
Área do conhecimento
Ciências da Saúde
Modalidade de acesso
Acesso aberto
Editora
Autores
Dantas, Arlen Andrie Araújo
Soares, Camila Raene Vicente
Orientador
Pereira, Hannaly Wana Bezerra
Coorientador
Resumo
A expressão “arbovirose” foi denominada na década de 1930, quando inúmeros vírus foram isolados em artrópodes (Arthropod Borne Viruses), na sua maioria, mosquitos. Os vírus Dengue, Zika e Chikungunya são, atualmente, considerados importantes problemas de saúde e de impacto socioeconômico, uma vez que estão ligados a surtos epidêmicos anuais. Este estudo teve como objetivo descrever os aspectos virológicos, epidemiológicos, clínicos e laboratoriais das arboviroses. Para o levantamento bibliográfico sobre a temática, foram feitas pesquisas utilizando bancos de dados eletrônicos como: PubMed, LILACS e SciELO, e foram selecionados artigos nas línguas portuguesa e inglesa, abrangendo o período entre 1994 a 2022. O diagnóstico laboratorial de infecção por arbovírus, pode ser realizado de duas maneiras, seja por meio da detecção direta do agente infeccioso ou pela detecção de anticorpos direcionados contra o vírus. O método de RT-PCR em tempo real é eficiente no diagnóstico das arboviroses, pois permite detectar os agentes patogênicos, minimizando a ocorrência das reações cruzadas e da baixa reação inespecífica. Isso, portanto, contribui para um diagnóstico mais preciso e um tratamento mais assertivo.
The term “arboviruses” was coined in the 1930s, when several viruses were isolated from arthropods (Arthropod Borne Viruses), mostly mosquitoes. Dengue, Zika and Chikungunya viruses are currently considered important health problems with socioeconomic impact, since they are linked to annual epidemic outbreaks. This study aimed to describe the virological, epidemiological, clinical and laboratory aspects of arboviruses. For the bibliographic survey on the subject, research was carried out using electronic databases such as: PubMed, LILACS and SciELO, and articles in Portuguese and English were selected, covering the period between 1994 and 2022. The laboratory diagnosis of arbovirus infection, can be performed in two ways, either through the direct detection of the infectious agent or through the detection of targets against the virus. The real-time RT-PCR method is efficient in the diagnosis of arboviruses, as it allows the detection of pathogenic agents, minimizing the occurrence of cross-reactions and low non-specific reaction. This, therefore, contributes to a more accurate diagnosis and a more assertive treatment
The term “arboviruses” was coined in the 1930s, when several viruses were isolated from arthropods (Arthropod Borne Viruses), mostly mosquitoes. Dengue, Zika and Chikungunya viruses are currently considered important health problems with socioeconomic impact, since they are linked to annual epidemic outbreaks. This study aimed to describe the virological, epidemiological, clinical and laboratory aspects of arboviruses. For the bibliographic survey on the subject, research was carried out using electronic databases such as: PubMed, LILACS and SciELO, and articles in Portuguese and English were selected, covering the period between 1994 and 2022. The laboratory diagnosis of arbovirus infection, can be performed in two ways, either through the direct detection of the infectious agent or through the detection of targets against the virus. The real-time RT-PCR method is efficient in the diagnosis of arboviruses, as it allows the detection of pathogenic agents, minimizing the occurrence of cross-reactions and low non-specific reaction. This, therefore, contributes to a more accurate diagnosis and a more assertive treatment
Palavras-chave
Arboviroses, Diagnóstico laboratorial, Aedes Aegypti