Avaliação da atividade antibacteriana do extrato etanólico de folhas e cascas de eucalyptus globulus labill. Frente à escherichia coli e staphylococcus aureus
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Data
2022
Tipo de documento
Artigo Científico
Título da Revista
ISSN da Revista
Título de Volume
Área do conhecimento
Ciências da Saúde
Modalidade de acesso
Acesso aberto
Editora
Autores
Miranda, Débora Marroni de
Santos, Haylander Francesco dos
Orientador
Rocha, Marina Pereira
Coorientador
Resumo
A resistência bacteriana aos antimicrobianos disponíveis tem se tornado um grave problema de saúde pública em todo o mundo, pois além da dificuldade no tratamento das doenças infecciosas, representa um grande ônus para o sistema público de saúde. O presente estudo visa avaliar a atividade antibacteriana dos extratos de folhas e cascas de Eucalyptus globulus Labill. frente à Escherichia coli e Staphylococcus aureus. Os extratos das folhas e cascas foram produzidos por maceração dinâmica com banho de ultrassom. A análise da atividade antibacteriana dos extratos foi realizada pelo método de disco difusão em Ágar Mueller-Hinton. Os resultados encontrados na análise dos dados do extrato etanólico demonstraram que Eucalyptus globulus apresenta atividade antibacteriana sobre Staphylococcus aureus, na concentração de 10 mg/mL, com diâmetros de halos de inibição de 10 mm e 9 mm, para folhas e cascas, respectivamente. Na análise dos dados para Escherichia coli, apenas o extrato etanólico das cascas de 10 mg/mL apresentou atividade antibacteriana, com halo de inibição de 18 mm. Os resultados obtidos neste trabalho amplia o conhecimento e embasa pesquisas futuras com o Eucalyptus globulus, porém, outros estudos devem ser realizados para investigar o potencial antibacteriano descrito neste trabalho para determinar a concentração inibitória mínima e adotar testes quantitativos.
Bacterial resistance to available antimicrobials has become a serious public health problem worldwide, as in addition to the difficulty in treating infectious diseases, it represents a great burden for the public health system. This study aims to evaluate the antibacterial activity of extracts from leaves and bark of Eucalyptus globulus Labill. against Escherichia coli and Staphylococcus aureus. The extracts of leaves and bark were produced by dynamic maceration with an ultrasound bath. The analysis of the antibacterial activity of the extracts was performed using the disk diffusion method in Mueller-Hinton Agar. The results found in the analysis of the ethanol extract data showed that Eucalyptus globulus has antibacterial activity on Staphylococcus aureus, at a concentration of 10 mg/mL, with diameters of inhibition halos of 10 mm and 9 mm, for leaves and bark, respectively. In the data analysis for Escherichia coli, only the 10 mg/mL ethanol extract of the bark presented antibacterial activity, with an inhibition halo of 18 mm. The results obtained in this work expand the knowledge and support future researches with Eucalyptus globulus, however, other studies must be carried out to investigate the antibacterial potential described in this work to determine the minimum inhibitory concentration and to adopt quantitative tests.
Bacterial resistance to available antimicrobials has become a serious public health problem worldwide, as in addition to the difficulty in treating infectious diseases, it represents a great burden for the public health system. This study aims to evaluate the antibacterial activity of extracts from leaves and bark of Eucalyptus globulus Labill. against Escherichia coli and Staphylococcus aureus. The extracts of leaves and bark were produced by dynamic maceration with an ultrasound bath. The analysis of the antibacterial activity of the extracts was performed using the disk diffusion method in Mueller-Hinton Agar. The results found in the analysis of the ethanol extract data showed that Eucalyptus globulus has antibacterial activity on Staphylococcus aureus, at a concentration of 10 mg/mL, with diameters of inhibition halos of 10 mm and 9 mm, for leaves and bark, respectively. In the data analysis for Escherichia coli, only the 10 mg/mL ethanol extract of the bark presented antibacterial activity, with an inhibition halo of 18 mm. The results obtained in this work expand the knowledge and support future researches with Eucalyptus globulus, however, other studies must be carried out to investigate the antibacterial potential described in this work to determine the minimum inhibitory concentration and to adopt quantitative tests.
Palavras-chave
Eucalyptus globulus Labill, Eucalipto, Staphylococcus aureus, Escherichia coli, Avaliação antimicrobiana, Disco-difusão