Avaliação do efeito deletério renal, hepático: em ratos submetidos à terapia com alendronato de sódio, estudo histológico.
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Data
2022
Tipo de documento
Artigo Científico
Título da Revista
ISSN da Revista
Título de Volume
Área do conhecimento
Ciências da Saúde
Modalidade de acesso
Acesso embargado
Editora
Autores
Junior, Caulino Elizio Cardoso
Orientador
Molina, Gustavo Otoboni
Coorientador
Resumo
Objetivo: Avaliar em modelo animal a excreção, quando o mesmo é submetido à terapia com bisfosfonato. Materiais e métodos: A pesquisa utilizou 20 ratas da linhagem Wistar, com idade média de 200 dias, peso corporal de 300 a 400 g. Decorridos 60 dias do início do experimento, os animais foram mortos para a dissecação dos rins e fígados para análise de efeito de excreção. Os espécimes foram imersos em formalina neutra temporada a 10% durante 24 horas para fixação. Os cortes foram corados com hematoxilina e eosina (H&E) pela técnica-padrão. Resultado: No fígado, 50% dos animais apresentaram efeitos deletérios, sendo fibrose portal (40%), necrose de hepatócito (20%), colestase biliar (40%), e degeneração hidrópica (100%), no rim, todos apresentaram efeitos, sendo necrose tubular (60%), e material proteináceo (100%). Conclusão: No presente estudo, de acordo com os resultados encontrados foi possível concluir que o uso prolongado de bisfosfonatos, pode provocar efeitos adversos consideráveis nos sistema renal e hepático. Porém com a escassez de estudos dos efeitos renais e hepáticos dessa classe de droga, sugere-se a realização de novos estudos.
Objective: To evaluate excretion in an animal model when it is submitted to bisphosphonate therapy. Materials and methods: The research used 20 female Wistar rats, with an average age of 200 days, body weight of 300 to 400 g. After 60 days from the beginning of the experiment, the animals were killed for the dissection of the kidneys and livers to analyze the excretion effect. The specimens were immersed in 10% season neutral formalin for 24 hours for fixation. Sections were stained with hematoxylin and eosin (H&E) using the standard technique. Result: In the liver, 50% of the animals had deleterious effects, being portal fibrosis (40%), hepatocyte necrosis (20%), biliary cholestasis (40%), and hydropic degeneration (100%), in the kidney, all had effects. , being tubular necrosis (60%), and proteinaceous material (100%). Conclusion: In the present study, according to the results found, it was possible to conclude that the prolonged use of bisphosphonates can cause considerable adverse effects on the renal and hepatic systems. However, with the scarcity of studies on the renal and hepatic effects of this class of drug, further studies are suggested.
Objective: To evaluate excretion in an animal model when it is submitted to bisphosphonate therapy. Materials and methods: The research used 20 female Wistar rats, with an average age of 200 days, body weight of 300 to 400 g. After 60 days from the beginning of the experiment, the animals were killed for the dissection of the kidneys and livers to analyze the excretion effect. The specimens were immersed in 10% season neutral formalin for 24 hours for fixation. Sections were stained with hematoxylin and eosin (H&E) using the standard technique. Result: In the liver, 50% of the animals had deleterious effects, being portal fibrosis (40%), hepatocyte necrosis (20%), biliary cholestasis (40%), and hydropic degeneration (100%), in the kidney, all had effects. , being tubular necrosis (60%), and proteinaceous material (100%). Conclusion: In the present study, according to the results found, it was possible to conclude that the prolonged use of bisphosphonates can cause considerable adverse effects on the renal and hepatic systems. However, with the scarcity of studies on the renal and hepatic effects of this class of drug, further studies are suggested.
Palavras-chave
Bisfosfonato, Alendronato, Lesão renal, Lesão hepática