A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in mice
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Data
2018
Tipo de documento
Artigo de Periodico
Título da Revista
ISSN da Revista
Título de Volume
Área do conhecimento
Ciências da Saúde
Modalidade de acesso
Acesso aberto
Editora
Autores
Reitz, Felipe
Salm, Daiana
Ludtke, Daniela Dero
Santos, Aureo dos
Traebert, Jefferson
Martins, Daniel Fernandes
Orientador
Coorientador
Resumo
Background: Preclinical studies measure withdrawal responses to evoking thermal and mechanical stimuli instead
of the more clinically important spontaneous pain.
New method: Therefore, we studied the effect of peripheral inflammation induced by intraplantar administration
of complete Freund’s adjuvant (CFA) in mice on the variability of temperature and bioimpedance as an index of
pain produced by inflammation. To this end, we initially determined mathematical scores based on changes in
temperature and bioimpedance (STB) for animals with an inflamed paw and compared these scores with
commonly used measures of inflammatory pain. We then pharmacologically validated the tool using dexamethasone.
Results: The STB analysis resembled the response found in the von Frey Hair (vFH) test. The CFA-induced increase
in STB and vFH tests were reversed by intraperitoneal administration of dexamethasone. The correlation
between the STB and vFH measurements showed a high correlation coefficient (R2=0.911, p < 0.001).
Comparison with existing method: Our results also demonstrated that CFA paw injection induced mechanical
hyperalgesia in mice and remained virtually unaltered during all time-points tested for 5 days, as measured with
vFHs. The administration of CFA into the paw induced a large increase in paw volume that was apparent 1 and 5
days after the injection. The CFA injection resulted in a significant (p<0.05) decrease in the response latency to
the heat stimulus, as evaluated on day 4 post-CFA injection.
Conclusions: The data presented here suggest that STB may provide a novel non-invasive approach for inflammatory
pain detection.
Palavras-chave
Behavioral test, Inflammatory pain, Pain measurements, Thermography