A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in mice

Reitz, Felipe; Salm, Daiana; Ludtke, Daniela Dero; Santos, Aureo dos; Traebert, Jefferson; Martins, Daniel Fernandes

A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in mice

dc.contributor.author	Reitz, Felipe
dc.contributor.author	Salm, Daiana
dc.contributor.author	Ludtke, Daniela Dero
dc.contributor.author	Santos, Aureo dos
dc.contributor.author	Traebert, Jefferson
dc.contributor.author	Martins, Daniel Fernandes
dc.coverage.spatial	Nova York	pt_BR
dc.date.accessioned	2019-11-22T16:52:53Z
dc.date.accessioned	2020-11-26T17:41:09Z
dc.date.available	2019-11-22T16:52:53Z
dc.date.available	2020-11-26T17:41:09Z
dc.date.issued	2018	pt_BR
dc.description.abstract	Background: Preclinical studies measure withdrawal responses to evoking thermal and mechanical stimuli instead of the more clinically important spontaneous pain. New method: Therefore, we studied the effect of peripheral inflammation induced by intraplantar administration of complete Freund’s adjuvant (CFA) in mice on the variability of temperature and bioimpedance as an index of pain produced by inflammation. To this end, we initially determined mathematical scores based on changes in temperature and bioimpedance (STB) for animals with an inflamed paw and compared these scores with commonly used measures of inflammatory pain. We then pharmacologically validated the tool using dexamethasone. Results: The STB analysis resembled the response found in the von Frey Hair (vFH) test. The CFA-induced increase in STB and vFH tests were reversed by intraperitoneal administration of dexamethasone. The correlation between the STB and vFH measurements showed a high correlation coefficient (R2=0.911, p < 0.001). Comparison with existing method: Our results also demonstrated that CFA paw injection induced mechanical hyperalgesia in mice and remained virtually unaltered during all time-points tested for 5 days, as measured with vFHs. The administration of CFA into the paw induced a large increase in paw volume that was apparent 1 and 5 days after the injection. The CFA injection resulted in a significant (p<0.05) decrease in the response latency to the heat stimulus, as evaluated on day 4 post-CFA injection. Conclusions: The data presented here suggest that STB may provide a novel non-invasive approach for inflammatory pain detection.	pt_BR
dc.format.extent	192 - 196	pt_BR
dc.identifier.issn	0165-0270	pt_BR
dc.identifier.uri	https://repositorio.animaeducacao.com.br/handle/ANIMA/2674
dc.language.iso	en_US	pt_BR
dc.relation.ispartofseries	308	pt_BR
dc.rights	Attribution-NonCommercial-NoDerivs 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/br/	*
dc.subject	Behavioral test	pt_BR
dc.subject	Inflammatory pain	pt_BR
dc.subject	Pain measurements	pt_BR
dc.subject	Thermography	pt_BR
dc.title	A non-reflexive method based on the variability of temperature and bioimpedance in measuring inflammatory hyperalgesia and analgesia in mice	pt_BR
dc.type	Artigo de Periodico	pt_BR
local.affiliation.ies	Unisul
local.author.curso	Produção Científica	pt_BR
local.author.unidade	Ânima	pt_BR
local.rights.policy	Acesso aberto	pt_BR
local.subject.area	Ciências da Saúde	pt_BR
local.subject.areaanima	Ciências Humanas	pt_BR