Atividade antibiofilme de scaffolds tridimensionais de nanocelulose bacteriana impregnados com clorexidina e antibióticos para uso na terapia endodôntica regenerativa
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Data
2023-06-16
Tipo de documento
Artigo Científico
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Área do conhecimento
Ciências da Saúde
Modalidade de acesso
Acesso embargado
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Autores
Ávila da Silva, Fernanda Keil Kressin
Orientador
Cava da Silveira, Josiane de Almeida
Coorientador
Resumo
O objetivo do estudo foi avaliar a atividade antibiofilme de scaffolds tridimensionais de nanocelulose bacteriana (BNC), modificados com um sistema de liberação lenta de fármacos, e incorporados com clorexidina 0,12% (BNC/CHX), amoxicilina 1% (BNC/AMOX) e clindamicina 1% (BNC/CLI). Membranas sem agente antimicrobiano compuseram o grupo controle (BNC/C). As membranas foram imersas em uma cultura multiespécie de Enterococcus faecalis, Actinomices naeslundii e Streptococcu sanguinis, servindo de substrato para o crescimento do biofilme. Após 24 horas, 7 e 15 dias, o valor médio de unidades formadoras de colônias (UFC) foi determinado. Os dados foram analisados pelos testes Kruskal-Wallis e post hoc Dunn (α=5%). No período de 24h, BNC/AMOX inibiu a formação de biofilme na membrana, comparada aos demais grupos (P < 0,05). Contudo, os valores de UFC aumentaram ao longo do período experimental, sem diferença significativa comparada à BNC/C (P > 0,05). Por outro lado, BNC/CHX permitiu a formação densa de biofilme já em 24h, a qual aumentou progressivamente ao longo do período experimental, com valores de UFC semelhante ao controle (P > 0,05). BNC/CLI inibiu significativamente a formação de biofilme comparada à BNC/C apenas no período de 24h (P < 0,05); nos demais períodos, os valores de UFC foram semelhantes (P > 0,05). Amostras das diferentes membranas foram observadas em Microscopia Eletrônica de Varredura e Microscopia Confocal de Varredura à Laser e ilustraram os resultados obtidos na análise quantitativa. Conclusão: BNC/AMOX demonstrou atividade antibiofilme eficiente contra E. faecalis, A. naeslundii e S. sanguinis em 24h. Contudo, o sistema de liberação lenta de fármacos não foi eficiente, visto que ao longo do período experimental todas as membranas permitiram a formação do biofilme multiespécie.
The aim of the study was to evaluate the antibiofilm activity of three-dimensional bacterial nanocellulose (BNC) scaffolds, modified with a slow drug release system, and incorporated with 0.12% chlorhexidine (BNC/CHX), 1% amoxicillin (BNC/AMOX) and 1% clindamycin (BNC/CLI). Membranes without antimicrobial agent composed the control group (BNC/C). Membranes were immersed in a multispecies culture of Enterococcus faecalis, A. naeslundii and Streptococcu sanguinis, serving as substrate for biofilm growth. After 24 hours, 7 and 15 days, the average value of colony forming units (CFU) was determined. Data were analyzed by Kruskal-Wallis and post hoc Dunn tests (α=5%). In the 24h period, BNC/AMOX inhibited the formation of biofilm on the membrane, compared to the other groups (P < 0.05). However, CFU values increased throughout the experimental period, with no significant difference compared to BNC/C (P > 0.05). On the other hand, BNC/CHX allowed dense biofilm formation as early as 24h, which progressively increased throughout the experimental period, with CFU values similar to the control (P > 0.05). BNC/CLI significantly inhibited biofilm formation compared to BNC/C only in the 24h period (P < 0.05); in the other periods, CFU values were similar (P > 0.05). Samples of different membranes were observed in MEV and Confocal and illustrated the results obtained in the quantitative analysis. Conclusion: BNC/AMOX demonstrated efficient antibiofilm activity against E. faecalis, A. naeslundii and S. sanguinis in 24h. However, the slow drug release system was not efficient, since throughout the experimental period all membranes allowed multispecies biofilm formation.
The aim of the study was to evaluate the antibiofilm activity of three-dimensional bacterial nanocellulose (BNC) scaffolds, modified with a slow drug release system, and incorporated with 0.12% chlorhexidine (BNC/CHX), 1% amoxicillin (BNC/AMOX) and 1% clindamycin (BNC/CLI). Membranes without antimicrobial agent composed the control group (BNC/C). Membranes were immersed in a multispecies culture of Enterococcus faecalis, A. naeslundii and Streptococcu sanguinis, serving as substrate for biofilm growth. After 24 hours, 7 and 15 days, the average value of colony forming units (CFU) was determined. Data were analyzed by Kruskal-Wallis and post hoc Dunn tests (α=5%). In the 24h period, BNC/AMOX inhibited the formation of biofilm on the membrane, compared to the other groups (P < 0.05). However, CFU values increased throughout the experimental period, with no significant difference compared to BNC/C (P > 0.05). On the other hand, BNC/CHX allowed dense biofilm formation as early as 24h, which progressively increased throughout the experimental period, with CFU values similar to the control (P > 0.05). BNC/CLI significantly inhibited biofilm formation compared to BNC/C only in the 24h period (P < 0.05); in the other periods, CFU values were similar (P > 0.05). Samples of different membranes were observed in MEV and Confocal and illustrated the results obtained in the quantitative analysis. Conclusion: BNC/AMOX demonstrated efficient antibiofilm activity against E. faecalis, A. naeslundii and S. sanguinis in 24h. However, the slow drug release system was not efficient, since throughout the experimental period all membranes allowed multispecies biofilm formation.
Palavras-chave
Biofilme multiespécie, Endodontia regenerativa, Nanocelulose bacteriana