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Navegando Ciências Biológicas- Licenciatura por Assunto "Aedes albopictus"
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Monografia Acesso fechado Avaliação da atividade larvicida de sais imidazólicos sobre Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)(2019) Gonçalves, Douglas ZelingerThe Aedes albopictus (Skuse, 1894) mosquito is considered a vector of medical importance because it has vector capacity in the laboratory for 22 arboviruses, including dengue, chikungunya and yellow fever viruses. The present study aimed to evaluate the larvicidal activity of the imidazolium salts (IS) C16MImMeS and C18MImCl against A. Albopictus larvae. For this, larvae of Ae. albopictus of 3rd final instar and/or 4th initial instar were exposed to 11 different concentrations (ranging from 0.5ppm to 10ppm) of C16MImMeS and C18MImCl. The bioassay was performed in triplicate, containing 20 larvae in each replicate and using purified water as control. It was conducted at a temperature of 25°C with a relative humidity of 80% (± 10%) in a climate chamber. Larval mortality was evaluated at 24 and 48 hours of exposure of the larvae to the solutions, being considered dead larvae that did not move when touched. For the results analysis, the variance test (ANOVA) was performed, with significant values of p≤0.05. Lethal concentrations, those that caused 50% mortality (LC50) were calculated by the Probit program. The IS C16MImMeS and C18MImCl showed larvicidal activity against A. albopictus at 24 and 48h exposure. At the lowest concentration (0.5ppm) of C16MImMeS and C18MImCl, mortality was 6.6 and 23.3%, at the highest concentration (10ppm) mortality was 85 and 100%, respectively. The 50% lethal concentrations (LC50) of IS C16MImMeS and C18MImCl at 48h exposure were 2.35ppm and 3.61ppm, respectively. Imidazolium salts C16MImMeS and C18MImCl have larvicidal potential against A. albopictus, being the first study to demonstrate the use of these IS to control A. albopictus. Further studies are necessary to understand its behavior in pupae and mosquito adulthood, its mechanism of action, and possible resistance factors.Monografia Acesso fechado Avaliação da eficácia de diferentes repelentes comerciais contra Aedes albopictus e Aedes aegypti(2019) Costa, Felipe Allan da SilvaThe use of repellents as a form of individual protection is stimulated to reduce the number of interactions between hosts and vectors and consequently the spread of pathogens. This study evaluated the efficacy of different commercially used repellents against Aedes albopictus and Aedes aegypti mosquitoes. Methodology: Three commercial repellent formulations containing DEET, Icaridina and IR3535 as active principle were evaluated. The test consisted of the application of 4mg of the product on the hands of volunteers and the introduction of hands in cages containing 20 female mosquitoes each. The sting attempts were counted for 10 minutes in all tests, and the repellents were evaluated 30 minutes, 2 hours and 5 hours after application. Results: The performance of the three formulations of repellents was similar in Aedes albopictus, reaching> 98% in the repellency indexes at all times. In the tests carried out with Aedes aegypti, the DEET content presented a low performance, reaching 42% in the 5 hour test repellency index, followed by repellents containing IR3535 and Icaridina, which obtained 66% and 77.5%, respectively. Conclusions: The formulation containing Icaridina obtained 100% in the index of repellency against Aedes albopictus, while no formulation offered 100% in the index of repellency against Aedes aegypti, during the times specified in the labels. Therefore, tested repellents containing DEET, Icaridina and IR3535 as an active principle offer the potential to reduce bites and not necessarily to provide full protection, allowing consumers to use unprotected insect repellents from several arboviruses.