Imersão em água termomineral reduz dor neuropática em camundongos e imunoconteúdo de BDNF e nos2 na medula espinal
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Data
2020-10-02
Tipo de documento
Tese
Título da Revista
ISSN da Revista
Título de Volume
Área do conhecimento
Ciências da Saúde
Modalidade de acesso
Acesso aberto
Editora
Autores
Brito, Rômulo Nolasco de
Orientador
Martins, Daniel Fernandes
Coorientador
Horewicz, Verônica Vargas
Resumo
Introdução: Nas últimas décadas, a imersão em águas termominerais (IATM)
também conhecido como balneoterapia (BT) ou termalismo tem sido utilizada para
promoção da saúde e no tratamento de distúrbios osteomusculares, processos
inflamatórios e crônicos. Estudos recentes demonstraram os efeitos de processos
crônicos, como no tratamento da dor lombar crônica, mas não analisaram os
mecanismos envolvidos no alívio da dor, em particular a dor neuropática (DN), comum
nesses indivíduos com dor lombar. Objetivo: este estudo investigou o efeito antihiperalgésico da BT em um modelo animal de dor neuropática. Métodos: Os animais
(camundongos swiss machos adultos 25-35g de peso corporal), foram operados por
meio de ligadura parcial do nervo isquiático, induzindo DN. O tratamento com imersão
em água termomineral (IATM) (35ºC) foi realizado em ambiente experimental
controlado; o filamento von Frey (vF) e o peso do corpo foram utilizados para avaliar
a hiperalgesia mecânica (dor neuropática); a força de agarrar da pata traseira foi
medida usando um dinamômetro digital; fator neurotrófico derivado do encéfalo
(BDNF) usando método ELISA, isoforma NOS neuronal (nNOS ou NOS1) e a isoforma
induzida (iNOS ou NOS2) foram quantificados por imunoeletroforese. Resultados:
Nossos achados mostram que a BT reduziu de forma efetiva a hiperalgesia mecânica,
preveniu a diminuição do peso corporal dos animais e aumento do imunoconteúdo de
BDNF e NOS2 na medula espinal, enquanto aumentou o imunoconteúdo de BDNF e
NOS1 na pele da pata. Não foram observados efeitos da BT ou da LPNI no
imunoconteúdo de TrkB na medula espinal e na pele da pata. O conjunto de
experimentos demonstra pela primeira vez um efeito analgésico da BT na água termal
da nascente natural de Santo Amaro da Imperatriz-Brasil em um modelo de dor
neuropática em murinos. Conclusão: O presente estudo fornece dados experimentais
in vivo importantes, baseados no modelo murino de dor neuropática, para estabelecer
os efeitos da BT na água termal de nascentes naturais de Santo Amaro da ImperatrizBrasil por abordagem metodológica, usando testes funcionais e análises bioquímicas.
Além disso, nossos estudos em animais fornecem evidências experimentais de efeitos
favoráveis da água de Santo Amaro da Imperatriz que podem ser utilizadas
clinicamente em pacientes com dor neuropática. No entanto, futuros ensaios clínicos
devem ser incentivados para testar a eficácia da BT em pacientes com dor
neuropática.
Introduction: In the last decades, immersion in thermal mineral waters (ATM) also known as balneotherapy (BT) or thermalism has been used for health promotion and in the treatment of musculoskeletal disorders, inflammatory and chronic processes. Recent studies have demonstrated the effects of chronic processes, such as in the treatment of chronic low back pain, but have not analyzed the mechanisms involved in pain relief, in particular neuropathic pain (DN), common in these individuals with low back pain. Objective: this study investigated the anti-hyperalgesic effect of BT in an animal model of neuropathic pain. Methods: The animals were operated using partial sciatic nerve connection, inducing DN. The treatment with immersion in thermomineral water (IATM) (35ºC) was carried out in a controlled experimental environment; the von Frey filament (vF) and body weight were used to assess mechanical hyperalgesia (neuropathic pain); the gripping strength of the hind leg was measured using a digital dynamometer; brain-derived neurotrophic factor (BDNF), neuronal NOS isoform (nNOS or NOS1), induced (iNOS or NOS2) were quantified by immunoelectrophoresis. Results: Our findings show that BT effectively reduced mechanical hyperalgesia, prevented the animals' body weight from decreasing and increased the BDNF and NOS2 immunocontent in the spinal cord, while increasing the BDNF and NOS1 immunocontent in the paw skin. No effects of BT or LPNI were observed on TrkB immunocontent in the spinal cord and paw skin. The set of experiments demonstrates for the first time an analgesic effect of BT in the thermal water of the natural spring of Santo Amaro da Imperatriz-Brasil in a model of neuropathic pain in murines. Conclusion: The present study provides important in vivo experimental data, based on the murine model of neuropathic pain, to establish the effects of BT on the thermal water of natural springs in Santo Amaro da Imperatriz-Brasil by methodological approach, using functional tests and biochemical analyzes. In addition, our animal studies provide experimental evidence of favorable effects of Santo Amaro da Imperatriz water that can be observed clinically in patients with neuropathic pain. However, future clinical trials should be encouraged to test the effectiveness of BT in patients with neuropathic pain
Introduction: In the last decades, immersion in thermal mineral waters (ATM) also known as balneotherapy (BT) or thermalism has been used for health promotion and in the treatment of musculoskeletal disorders, inflammatory and chronic processes. Recent studies have demonstrated the effects of chronic processes, such as in the treatment of chronic low back pain, but have not analyzed the mechanisms involved in pain relief, in particular neuropathic pain (DN), common in these individuals with low back pain. Objective: this study investigated the anti-hyperalgesic effect of BT in an animal model of neuropathic pain. Methods: The animals were operated using partial sciatic nerve connection, inducing DN. The treatment with immersion in thermomineral water (IATM) (35ºC) was carried out in a controlled experimental environment; the von Frey filament (vF) and body weight were used to assess mechanical hyperalgesia (neuropathic pain); the gripping strength of the hind leg was measured using a digital dynamometer; brain-derived neurotrophic factor (BDNF), neuronal NOS isoform (nNOS or NOS1), induced (iNOS or NOS2) were quantified by immunoelectrophoresis. Results: Our findings show that BT effectively reduced mechanical hyperalgesia, prevented the animals' body weight from decreasing and increased the BDNF and NOS2 immunocontent in the spinal cord, while increasing the BDNF and NOS1 immunocontent in the paw skin. No effects of BT or LPNI were observed on TrkB immunocontent in the spinal cord and paw skin. The set of experiments demonstrates for the first time an analgesic effect of BT in the thermal water of the natural spring of Santo Amaro da Imperatriz-Brasil in a model of neuropathic pain in murines. Conclusion: The present study provides important in vivo experimental data, based on the murine model of neuropathic pain, to establish the effects of BT on the thermal water of natural springs in Santo Amaro da Imperatriz-Brasil by methodological approach, using functional tests and biochemical analyzes. In addition, our animal studies provide experimental evidence of favorable effects of Santo Amaro da Imperatriz water that can be observed clinically in patients with neuropathic pain. However, future clinical trials should be encouraged to test the effectiveness of BT in patients with neuropathic pain
Palavras-chave
Balneoterapia, dor crônica, hidroterapia, águas termais, lesão nervosa