Avaliação da atividade antinociceptiva e antiartrítica de extrato bruto hidroalcoólico de Citrus reticulata (Ponkan) em camundongos
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Data
2014
Tipo de documento
Dissertação
Título da Revista
ISSN da Revista
Título de Volume
Área do conhecimento
Ciências da Saúde
Modalidade de acesso
Acesso aberto
Editora
Autores
Schneider, Adriele Cristina Alves
Orientador
Piovezan, Anna Paula
Coorientador
Resumo
O presente estudo deve como objetivo avaliar o efeito antinociceptivo de um extrato hidroalcoolico a partir das cascas da fruta Citrus reticulata (EBCR), popularmente conhecida como ponkan, em diferentes modelos de nocicepcao e em modelo de AR induzido por Complete Freund¿fs Adjuvant (CFA). A artrite reumatoide (AR) e uma doenca que tem como principal sintoma a dor, os medicamentos utilizados no tratamento levam a diversos efeitos colaterais, sendo importante a busca por medicamentos fitoterapicos com menos efeitos adversos. Os modelos de nocicepcao aguda tais como o teste do glutamato, da formalina e da carragenina serviu para uma triagem preliminar da capacidade do extrato de planta em promover acao antinociceptiva, ja que a dor e o principal sintoma a ser eliminado durante a AR. Os experimentos foram realizados em camundongos da linhagem Swiss, machos. Antes de cada experimento os animais eram aclimatados por pelo menos uma hora. A nocicepcao foi avaliada atraves do comportamento de lamber a pata e pela alodinia mecanica, avaliada pelo teste de Von Frey (forca de dobragem de 0,6g); a medida do edema foi obtida atraves de micrometro, sendo os resultados expressos como a diferenca entre a espessura da pata traseira direita (em ¿Êm) antes e apos a administracao do agente inflamatorio. Na analise do teor do flavonoide naringenina, foi encontrado 0,29 mg do flavonoide por grama do extrato, quantidade diferente as encontradas em outros estudos. O extrato nas doses de 10, 100 e 300mg/Kg nao causaram efeito sobre a primeira fase da formalina (dor neurogenica), porem apresentou efeito na segunda fase (dor inflamatoria). O EBCR nao apresentou efeito no teste do glutamato. No modelo de carragenina todas as doses apresentaram efeito nociceptivo, tanto no tratamento profilatico como terapeutico. No modelo do CFA a dose de 30mg/Kg foi a que apresentou melhor efeito apos uma hora do tratamento. No terceiro dia do CFA foi avaliado a acao dos sistemas opioidergico e adenosinergico, seus antagonistas naloxona e cafeina, respectivamente, reverteu a acao do extrato, indicando uma possivel acao desses sistemas sobre o EBCR. O efeito antiedematogenico do EBCR so foi visto no tratamento terapeutico no modelo de carragenina. Em conjunto os resultados indicaram uma importante acao antinociceptiva do EBCR, inclusive em modelo de AR.
The present study aimed to evaluate the analgesic effect of a hydroalcoholic extract from the barks of the fruit Citrus reticulata (EBCR), popularly known as ponkan in different models of nociception and AR model induced by Complete Freund's Adjuvant (CFA). Rheumatoid arthritis (RA) is a disease whose main symptom was pain, medicines used to treat it can lead on many side effects, for this is important to search for herbal medicines with fewer adverse effects. Models of acute nociception such as testing of glutamate, formalin and carrageenan were used for preliminary screening of the ability of plant extracts to promote antinociceptive, since pain is the main symptom being eliminated during AR. The experiments were performed in male mice of the Swiss strain. Before each experiment the animals were acclimated for at least an hour. Nociception was assessed using the paw licking behavior, and the mechanical allodynia, was assessed by von Frey test (bending force of 0.6 g); measurement of edema was obtained by micrometer, and the results were expressed as the difference between the thickness of the right hind paw (in ìm) before and after administration of the inflammatory agent. On the analysis of the naringenin flavonoid, was found 0.29 mg per gram of extract, different amount was found in other studies. The extract, at doses of 10, 100 and 300mg/kg, did not show effect on the first phase of formalin (neurogenic pain), however, it was effective in the second phase (inflammatory pain). The EBCR had no effect on the glutamate test. In the carrageenan model all doses had nociceptive effect, both in the prophylactic and therapeutic treatment. In the CFA model 30mg/Kg dose showed the best effect after one hour of treatment. On the third day of the CFA was evaluated the action of opioid systems and adenosinergic, their antagonists naloxone and caffeine, respectively, reversed the action of the extract, indicating a possible effect of these systems on the EBCR. About the antiedematogenic effect was only seen in the therapeutic treatment in the carrageenan model. Together, the results indicated a significant antinociceptive action, including in the AR model.
The present study aimed to evaluate the analgesic effect of a hydroalcoholic extract from the barks of the fruit Citrus reticulata (EBCR), popularly known as ponkan in different models of nociception and AR model induced by Complete Freund's Adjuvant (CFA). Rheumatoid arthritis (RA) is a disease whose main symptom was pain, medicines used to treat it can lead on many side effects, for this is important to search for herbal medicines with fewer adverse effects. Models of acute nociception such as testing of glutamate, formalin and carrageenan were used for preliminary screening of the ability of plant extracts to promote antinociceptive, since pain is the main symptom being eliminated during AR. The experiments were performed in male mice of the Swiss strain. Before each experiment the animals were acclimated for at least an hour. Nociception was assessed using the paw licking behavior, and the mechanical allodynia, was assessed by von Frey test (bending force of 0.6 g); measurement of edema was obtained by micrometer, and the results were expressed as the difference between the thickness of the right hind paw (in ìm) before and after administration of the inflammatory agent. On the analysis of the naringenin flavonoid, was found 0.29 mg per gram of extract, different amount was found in other studies. The extract, at doses of 10, 100 and 300mg/kg, did not show effect on the first phase of formalin (neurogenic pain), however, it was effective in the second phase (inflammatory pain). The EBCR had no effect on the glutamate test. In the carrageenan model all doses had nociceptive effect, both in the prophylactic and therapeutic treatment. In the CFA model 30mg/Kg dose showed the best effect after one hour of treatment. On the third day of the CFA was evaluated the action of opioid systems and adenosinergic, their antagonists naloxone and caffeine, respectively, reversed the action of the extract, indicating a possible effect of these systems on the EBCR. About the antiedematogenic effect was only seen in the therapeutic treatment in the carrageenan model. Together, the results indicated a significant antinociceptive action, including in the AR model.
Palavras-chave
Dor - Tratamento, Edema, Opióides, Frutas cítricas - Pesquisa, Artrite reumatóide