Programa de Pós-Graduação em Ciências da Saúde
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Navegando Programa de Pós-Graduação em Ciências da Saúde por Assunto "Ácidos graxos poli-insaturados ômega-3"
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Dissertação Acesso embargado Suplementação preventiva de ômega-3 diminui comportamento do tipo dor e citocinas pró-inflamatórias em um modelo animal de Síndrome da Dor Regional Complexa do tipo I(2020) Galassi, Taynah de OliveiraIntroduction: Complex regional pain syndrome type I (CRPS-I) is characterized as a chronic and disabling clinical condition that poorly responds to different treatments. The role of omega-3 polyunsaturated fatty acids in the prevention and treatment of inflammatory disorders is well described in the literature. Despite the proven anti-inflammatory effects of omega-3, there are few studies in the literature evaluating its therapeutic benefits in different types of pain. Aim: We evaluated the potential anti-inflammatory and antihyperalgesic effects of preventive omega-3 supplementation in an animal model of CRPS-I in mice. Methods: Female mice were supplemented for 30 days with omega-3 before the induction of the CRPS-I model and 14 days after. Mechanical hyperalgesia was daily evaluated from the 4th day after ischemia and reperfusion (IR) procedure until the 14th day. The animals were also submitted to the pain-related cognitive ability test and spontaneous locomotor activity analysis. In addition, other female mice were supplemented for 30 days with omega-3 and then subjected to the IR procedure. Twenty-four hours later, assisted painless death was conducted and samples of the spinal cord and right posterior paw muscle were taken to measure the proinflammatory cytokine TNF and IL-1β concentrations. Results: Preventive omega-3 supplementation elicited antihyperalgesic effects and reduced the concentrations of proinflammatory cytokines TNF in the spinal cord and IL-1β in the paw muscle of mice subjected to IR. No changes were found in the spontaneous locomotion of animals supplemented with omega-3. Conclusion: Preventive omega-3 supplementation was effective in reducing mechanical hyperalgesia, as well as producing anti-inflammatory effects reducing proinflammatory cytokine concentrations. The 30-day supplementation at the tested dose was safe and effective in the CRPS-I animal model and presented no sedative effects.